The pH value was measured in the same muscle (raw and cooked).
For raw samples, 1.0 cm-slice was separated from the end part of
the muscle; and for cooked ones, 1.0 cm-slice was excised from the
middle part. Slurries (5 g of sample: 25 ml of distilled water standardized
at pH 7) were prepared with a laboratory blender (StomacherTM,
Colworth, UK). The pH measurement was performed in duplicate
with a pH-meter (Thermo Orion 710A+, Beverly MA, USA) equipped
with a combination pH electrode (Thermo Orion Model 8102BN ROSS
Electrode, Beverly MA, USA) and a ATC-Probe (Thermo Orion, Beverly
MA, USA).