Inorganic carbon (CO2) was used as the carbon source in photoautotrophic cultivation, while organic carbon (sucrose) was used in heterotrophic cultivation. Mixotrophic cultivation,
which means the microalgae could undergo photosynthesis and simultaneously use both organic (fructose, glucose, glycerol, sucrose, and xylose) and inorganic carbon (CO2) as carbon
sources, was also investigated in this study. The effects of these cultivation conditions on microalgae growth and lipid production were investigated.