Exploration of Perennial Plants in Nature Trail at Khao Bunjob Waterfall, Khao Khitchakut National Park, Chanthaburi. Studies in the period January 2015 - April 2015. The nature trail is classified as a tropical rainforest. Use sampling plots of size (40 x 40) meters The number 6 plots. A survey and data collection perennial with circumference levels since 15 cm or above. And the temple at an altitude of the ground 1.30 meters. The survey found the tree 775 tree. This study found 775 tree specimens which categorized as 46 species, in 33 families. The most common families DIPTEROCARPACEAE, and EUPHORBIACEAE families. From the analysis the value of diversity found the most frequency tree value is Jiknomyan is up 3.26 percent Shows that species is the most dominant. The plant with the density is the most percentage namely Jiknomyan equal 10.32.