Chitosan derived from crab shell with a molecular weight of 684 kDa
and deacetylation degree of ∼85% was purchased from Sigma
(Germany). ZEO (98%) was purchased from Magnolia Co (IRAN).
Pentasodium triphosphate (TPP), acetic acid glacial and sodium hydroxide
were purchased from Merck (Germany). All chemicals used
were of analytical grade and used as received. B. cinerea (IRAN 1304C)
was obtained from the Iranian Research Institute of Plant Protection
(IRIPP).