Prepare the standard dilution series in the U-shaped plate. Prepare the samples and add
125 µl to the U-shaped plate.
3. Add 125 µl of the diluted tracer to the 125 µl of standards and samples in the U-shaped
plate. Gently mix by pipetting.
4. Cover the tray and tap the tray to eliminate any air bubbles. Be careful not to splash
liquid onto the cover.
5. Incubate U-shaped plate for 1 hour at room temperature.
6. Wash the microtiter plate coated with HSA-MGO 4 times with wash buffer using a plate
washer or as follows*:
a. Carefully remove cover, avoid splashing.
b. Empty the plate by inverting plate and shaking contents out over the sink, keep
inverted and tap dry on a thick layer of tissues.
c. Add 200 μl of wash buffer to each well, wait 20 seconds, empty the plate as described
in 6b.
d. Repeat the washing procedure 6b/6c three times.
e. Empty the plate and intensively tap on thick layer of tissues. Tap the plate as dry as
possible
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