Millipede manure
50–100 mg was extracted twice with methanol (50 %) in a
water bath (95 C, 10 min). The pooled extract was made
up to 10 ml, an aliquot (0.5 ml) was mixed with distilled
water (0.5 ml) and treated with Na2CO3 in NaOH (0.1 N,
5 ml). After incubation (10 min), Folin–Ciocalteus reagent
(0.5 ml) was added and optical density was read (725 nm)
with tannic acid as standard. Organic carbon in manure
(50–150 mg) was oxidized into CO2 by K2Cr2O7 (1 N). The
excess K2Cr2O7 after oxidation was titrated against
(NH4)2SO4FeSO46H2O (0.5 N) with diphenylamine indicator.
For the total nitrogen estimation, manure samples
(100 mg) were digested with catalytic mixture (1 g) and
concentrated sulphuric acid (10 ml). The digest was diluted
with distilled water (up to 100 ml in standard flask), aliquot
(10 ml) was distilled in micro-Kjeldahl apparatus, released
ammonia was absorbed into boric acid (2 %, 10 ml) and
titrated against HCl (0.01 N) with mixed indicator.
Millipede manure
50–100 mg was extracted twice with methanol (50 %) in a
water bath (95 C, 10 min). The pooled extract was made
up to 10 ml, an aliquot (0.5 ml) was mixed with distilled
water (0.5 ml) and treated with Na2CO3 in NaOH (0.1 N,
5 ml). After incubation (10 min), Folin–Ciocalteus reagent
(0.5 ml) was added and optical density was read (725 nm)
with tannic acid as standard. Organic carbon in manure
(50–150 mg) was oxidized into CO2 by K2Cr2O7 (1 N). The
excess K2Cr2O7 after oxidation was titrated against
(NH4)2SO4FeSO46H2O (0.5 N) with diphenylamine indicator.
For the total nitrogen estimation, manure samples
(100 mg) were digested with catalytic mixture (1 g) and
concentrated sulphuric acid (10 ml). The digest was diluted
with distilled water (up to 100 ml in standard flask), aliquot
(10 ml) was distilled in micro-Kjeldahl apparatus, released
ammonia was absorbed into boric acid (2 %, 10 ml) and
titrated against HCl (0.01 N) with mixed indicator.
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