The concentrate samples were diluted in a ratio of 1:10with twice distilled water. A 0.4 ml of diluted sample was mixed with 3.6 ml of corresponding buffers and allowed to equilibrate for 15 min at room temperature.
The concentrate samples were diluted in a ratio of 1:10with twice distilled water. A 0.4 ml of diluted sample was mixedwith 3.6 ml of corresponding buffers and allowed to equilibrate for 15 min at room temperature.