yeast Saccharomyces cerevisiae was employed. After six days of saccharification
mycelial mat of Aspergillus niger was removed under aseptic conditions and 10% of Saccharomyces cerevisiae
culture was added to all the flasks. The process was carried out for a period of six days at 300
C. During the
fermentation process every 24 hours samples were taken for the estimation of bioethanol (25). .