As another
control, target cells were cultured with 9.5 mL of
medium alone (NP, no plasma). In all cases, inocula or
NHP were removed after 24 h and the cells washed thoroughly
prior to suspension in 9.5 mL of medium, as described
[7]. Cells were cryopreserved for analysis prior to
and after PHA stimulation (time 0) and at 1, 4, 7 and 10
d.p.i., unless otherwise indicated. In addition, cells were
collected at each of the above time points to determine
cell phenotype and apoptosis (see below).