Details of the manipulation and purification of the
samples have been published before [13,14]. Briefly, the
most superficial layers of the samples were cut with a
bladder and discarded, and accurately weighed parts of
tissues were homogenized with anhydrous sodium sulfate. After the addition of internal standards (free FAs
12:0 for dolphins and 13:0 for turtles) and an acidic
solution of methanol (1 N H2
SO4
), the headspace of the
tubes were filled with pure N2
, the content mixed with
a vortex and the tubes placed in an oven at 60–80°C
for several hours, shaking the tubes occasionally.
FAMEs were extracted with n-hexane after adding
distilled water. Optimal conditions of transmethylation
of the FAs and reproducibility tests were checked and
performed during the development of the method
[13,14].
Details of the manipulation and purification of thesamples have been published before [13,14]. Briefly, themost superficial layers of the samples were cut with abladder and discarded, and accurately weighed parts oftissues were homogenized with anhydrous sodium sulfate. After the addition of internal standards (free FAs12:0 for dolphins and 13:0 for turtles) and an acidicsolution of methanol (1 N H2SO4), the headspace of thetubes were filled with pure N2, the content mixed witha vortex and the tubes placed in an oven at 60–80°Cfor several hours, shaking the tubes occasionally.FAMEs were extracted with n-hexane after addingdistilled water. Optimal conditions of transmethylationof the FAs and reproducibility tests were checked andperformed during the development of the method[13,14].
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