In brief, 50 ml of the supernatant above obtained (diluted if necessary) were mixed with 3 ml of freshly prepared FRAP reagent and the reaction mixtures incubated at 37 C for 30 min.
In brief, 50 ml of the supernatant above obtained (diluted ifnecessary) were mixed with 3 ml of freshly prepared FRAP reagentand the reaction mixtures incubated at 37 C for 30 min.