2.5. Microarrays data analysis
Signal processing was performed using vector oligonucleotide data to correct the relative amount of DNA present in each spot. At this step, low nucleotide signals (lower than 3 times the background level) were excluded from the analysis. After correction, signals were normalized by dividing each gene expression by the median value of the array. Data were subsequently analysed using statistical GeneANOVA software (Didier et al., 2002) and gene expression was significantly different when Pb0.01.
2.6. Data mining
Rainbow trout sequences originating from INRA Agenae (Govoroun et al.,2006) and USDA (Rexroad et al., 2003) and EST sequencing programs were used to generate publicly available contigs (http://public-contigbrowser.sigenae. org:9090/index.html). The 4th version (om.4) was used for BlastX (Basic Local Alignment Search Tool) comparison (http://www.ncbi.nlm.nih.gov/blast/). The score of each alignment was retrieved after performing a Blast X comparison.
Biological signification of data (biochemical pathways, cellular processes…) was illustrated using Kyoto Encyclopedia of Genes and Genomes (KEGG) as bioinformatics resource (http://www.genome.jp/kegg/).