This study showed excellent activity of makrut leaf oil and makrut oil against many respiratory bac- teria at the various activity levels. The results of disc diffusion were not highly correlated with MIC and MBC. Disc diffusion is a screening method usually used as a preliminary check for antibacterial activity prior to the more detailed study in liquid medium to determine MIC and MBC. There are many factors that may affect the results of disc-diffusion test, e.g., volume of essential oil placed on paper discs, thickness of agar, diffusion ability of oil in agar, and variation of essential oil concentration. Therefore, disc-diffusion method is not suitable for comparison the efficacy of essential oil. The broth microdilution method measures the strength of antibacterial activity and better be used for comparison.
This study showed excellent activity of makrut leaf oil and makrut oil against many respiratory bac- teria at the various activity levels. The results of disc diffusion were not highly correlated with MIC and MBC. Disc diffusion is a screening method usually used as a preliminary check for antibacterial activity prior to the more detailed study in liquid medium to determine MIC and MBC. There are many factors that may affect the results of disc-diffusion test, e.g., volume of essential oil placed on paper discs, thickness of agar, diffusion ability of oil in agar, and variation of essential oil concentration. Therefore, disc-diffusion method is not suitable for comparison the efficacy of essential oil. The broth microdilution method measures the strength of antibacterial activity and better be used for comparison.
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