The present study was carried out to evaluate the antimicrobial activity of clove oil extracted from clove. Screening for antibacterial and antifungal of clove oil was done using agar well diffusion. Four Bacterial and ten fungal species were taken. According to the result clove oil was more effective against Bacteria as compared to Fungi. We can conclude by this study that clove oil works better as antibacterial. It was also observed that by increasing concentration of clove oil the growth of fungi was decreased.