Microbial amylases have successfully replaced chemical hydrolysis of starch in starch processing industries. Fungal amylase is preferred for use in formulations for human or animal consumption involving applications under acidic conditions and around 37°C (Gupta et al., 2003). Among a large number of non-pathogenic microorganisms capable of producing useful enzymes, filamentous fungi are particularly interesting due to their easy cultivation and high production of extracellular enzymes of large industrial potential. Today, a large number of microbial α-amylases are marketed with applications in different industrial sectors and they have almost completely replaced chemical hydrolysis of starch in starch processing industry (Pandey et al., 2000; Patel et al., 2005). Each application requires unique pro-perties with respect to specificity, stability, temperature and pH dependence. These applications require enzymes with high activity and thermostability. There are many advantages of using thermostable enzymes, such as an increased reaction rate and decreased conta-mination risk through the use of high temperatures (Metin et al., 2010). This study reports the partial purification and characterization of extracellular amylases from four different Aspergillus species.