The PCR reaction mixture contained heat-lysed bacteria, 10 pmole of each primer, 0.2 mM dNTPs each, and 2.5 U Bio Tag DNA polymerase (Bioman, Taipei, Taiwan) in a volume of 50 l 1X PCR buffer from the manufacturer
The PCR reactionmixture contained heat-lysed bacteria, 10 pmoleof each primer, 0.2 mM dNTPs each, and 2.5 U BioTag DNA polymerase (Bioman, Taipei, Taiwan) in avolume of 50 l 1X PCR buffer from the manufacturer