2.8. Bagasse pretreatments
2.8.1. Physical pretreatment
2.8.1.1. Milling pretreatment. Ten grams of chipped and
ground bagasse were put into a 250 mL Erlenmeyer flask. It
was then moistened with distilled water; the flasks were incubated for 2 h at room temperature. The solid material was then
mixed vigorously with 100 mL distilled water for extraction of
soluble reducing sugars, then filtered with a cheese cloth to separate the contents into liquid and solid parts.