Systematic in vitro studies focusing on the effect
of different forms of oilseeds on the BH process are
still lacking. Recently, Hoffmann et al. (2013) determined
the effect of in vitro incubation of several oilseeds
and forms for 24 h on pattern of short
(≤C14), medium (C15–C17) and long-chain (≥C18)
FA. Based on this approach, the central focus of the
present in vitro study was directed to the effect of
these oilseeds and provided either as full-fat
crushed oilseed or as free oil, on dynamics of BH,
with main emphasis on long-chain (≥C18) FA and
BH intermediates (including CLA and VA). Linseed
and sunflower seed known as oilseeds with high
contents of LNA and LA, respectively, were used to
determine the dynamics of BH in comparison with
soya beans and rapeseed. Effects of the BH process
on FA pattern were determined after 0, 6, 12 and
24 h of in vitro incubation with rumen liquor. For
all oilseeds, changes in DB contents during BH were
determined. Potential differences both in the degree
of BH and production of BH intermediates as influenced
by the source and the physical form of the
different assay oilseeds were assessed.