India is world's second largest fruit and vegetable producer and encounters waste close t o25% worth of produce. It is also significant that 40% of loses occur at postharvest and processing levels while in industrialized countries loses happen only at retail and consumer levels. In recent times ,agricultural wastes have been made to use in biotechnological processes such as production of value add decompound sand substrates for microbial isolation. These help in disposal problems which other wise lead to pollution. Oil cakes from different oil extraction industries have been utilized for fermentative lipase production mainly because ;the residual oil content serves as inducer for lipase production. During the present study-groundnut cake ,rice bran ,wheat bran, neem cake, and coconut cake were tested for fermentative lipase production. Further ,development t of proper physical parameters for bioprocess and partial purification of lipase was performed.