of the month, in early/mid morning of each collection day.
All blood samples were examined for the presence of circulating
MF (see Section 2.3). Health observations of all the
dogs were recorded at each blood sampling throughout the
study.
Extent efficacy (EE) and intensity efficacy (IE) were calculated
as the proportion of dogs free of microfilariae and
the reduction in the number of MF in dogs starting from
the application of the drug till the end of the observation
period, respectively (Fok et al., 2010).