Exploration of Perennial Plants in Nature Trail at Khao Bunjob Waterfall, Khao Khitchakut National Park, Chanthaburi Province. Objective have to explore the types and amounts of tree species. Conducted during January - April 2558. Placing the sample size is 40x40 metres. The study sample Consisted of 6 plots cover the nature study path.Survey and data Collection perennial with Circumference in height from 15 centimeter or more .And measured at a height of 1.30 meters from the ground. The survey found 775 perennial plant tree were Classified as 46 species 33 families. The analysis for the diversity found the plant with most frequency values is the (Barringtonia macrostachya Hassk.) (Archidendron quocense Nielsen.) and (Walsura robusta Roxb).The species has a frequency equal to 3.26 percent.It also found the plants with the most prominent is (Barringtonia macrostachya Hassk.)14.08 percent (Archidendron quocense Nielsen.)