2.2. Materials used for prostheses and medical devicesFour different materials used for prostheses and medical deviceswere tested for sensitivity towards biofilm formation. Three of them arecommonly used for prostheses and medical devices: polyethylene,polytetrafluoroethylene and silicone rubber. Glass was added becauseof its highly hydrophilic nature compared to the other three materials.Polyethylene and polytetrafluoroethylene were obtained commerciallyas sheets with a thickness of 0.2 mm and 0.5 mm, respectively. Siliconerubber (M511 Maxillofacial Silicone System, Technovent Ltd., SouthWales, UK) for facial prostheses was used to prepare 60x60x1.5 mmsheets as described previously (Ariani et al., 2015). All polymers werecut into pieces of 10x10 mm. In addition, 10x10 mm glass slides with athickness of 1 mm were used. The materials were sterilized with 70%ethanol and dried under sterile conditions in a laminar airflow hood.