In this study, a polyethylene (PE) packaging material that contained nano-Ag, nano-TiO2, nano-SiO2, and attapulgite was prepared and its effect on storage stability of mushrooms (Flammulina velutipes) was investigated.The results showed that the nanocomposite-based packaging material (Nano-PM) regulated oxygen and carbon dioxide level, eliminated ethylene and inhibited the growth of microbes, which is a benefit on preservation quality of mushrooms, compared to the normal PE material (Normal-PM).