Another major milestone in the identification of actinomycetes was the assimilation of carbon by actinomycetes. Test includes ten carbon sources which are sterilized by membrane filtration method. Almost all the strains have shown very luxuriant growth. Some of the strains MB1, MB2, MB3, and MB5 have shown less growth. Pandey et al., (2005) showed that for the optimum production of antibiotics certain carbon sources are required. In that study the author and coworkers also suggested that pH might play an important factor for the production of antibiotics by actinomycetes. pH test done on all the 15 atrains has shown positive results.