Casein agar medium was prepared, sterilized and poured into
petri plates. The culture was incubated at 37 C at 24 h. The test
culture was streaked on the medium and incubated. Due to the
production of casienase enzyme by bacterium, medium surrounding the colony become clear since the exoenzymes has converted
larger protein molecules into smaller molecules i.e. amino acid,
which makes then invisible.