The total lipids contained in fried chicken fillets were
extracted according to the method by Folch, Lees, and Sloane-
Stanley (1957) with minor modification. Briefly, 15 g of crushed
chicken fillets sample was poured into a round bottom flask,
followed by addition of 60 mL chloroform-methanol (1:1, v/v)
and homogenization for 1.5e2.0 min. Successively, the mixture
was reflux-heated in a water bath at 60 C for 1 h and then
cooled to room temperature. After being filtered through a fluted
filter paper, the resultant filtrate was collected and dried under
reduced pressure using a vacuum evaporator. Then the fat obtained
was resolved in 25 mL petroleum ether, and mixed with
5 g sodium sulfate anhydrous to further remove the residual
solvent. Subsequently, the mixture was separated by centrifugation
at 3000 g for 5 min and the supernatant was collected,
evaporated and dried at 105 C in succession. The residue was
then weighed and stored at 20 C.