The supernatant from cultures of Bacillus sp. SMIA-2 grown in
sugarcane bagasse and corn steep liquor (120 h) contained
1.01 mg mL-1 of protein. The proteins may partly have originated from
the complex lignocellulosic biomass or from cells following lysis, and
may also constitute different enzymes, secreted to digest the
substrate. While expensive purification procedures may be required
for their removal, most industrial enzymes are consumed in the
presence of impurities that originate from culture supernatants. Thus,
the cellulases secreted by Bacillus sp. SMIA-2 were not purified,
beyond ammonium sulfate precipitation followed by dialysis.