To assure that soluble fractions of buffer Z are not contaminated with cytosolic proteins, the enzymatic activity of succinic dehydrogenase (marker of cytoplasmic proteins) was checked in previous preparations of OMPs from E. coli and Salmonella spp
To assure that soluble fractions of buffer Z are not contaminated withcytosolic proteins, the enzymatic activity of succinic dehydrogenase(marker of cytoplasmic proteins) was checked in previous preparationsof OMPs from E. coli and Salmonella spp