ttigh cell density fermentation production of recombinant human growth hormone was studied in ILs'cheri-
chia co/i.The results showed that by using glycerol instead of glucose as carbon source and feeding in a
specific fed-batch mode,by controlling the stirrer speed and keeping the dissolved oxygen level at 20-25¢~:
saturation,cell density increases from 38.6 to 118.8 g/l,the production of r-hGH remained high and the
fermentation time was reduced from 16 to 10h. © 1998 Published by Elsevier Science Ltd. All rights
reserved
Kcywords: Human growth hormone, high cell density fermentation, Escherichia coil, glycerol.