It is well known that the process of anaerobic degrade
organic matter can be divided into acid production phase and
the methane production phase. Therefore, it was crucial to create
the best environment conditions for hydrolysis acidification
bacteria and methanogen bacteria in one reactor. The integrated
two-phase anaerobic reactor can effectively separate acid production
phase and methane production phase. The effective volume
ratio between the two reaction zones was 1:2. And the HRT
ratio between the two reaction zones was 1:2. The soybean protein
wastewater was hydrolyzed and acidulated in the reaction
zone I. A large amount of organism was decomposed into volatile
fatty acid which could increase the substrate concentration
for the methanogenesis process. At the same time, it is unlikely
that a complete separation of phases (acidogenic and methanogenic)
occurred in the integrated two-phase anaerobic reactor
because biogas production was observed in the reaction zone I
and II.