A choice of differential characteristics between strains
TA 17, 23 and 24 and other bacteria, either oxalate oxidizers
or yellow pigmented “Pseudomonads” is given at
Table 1. 180 features, comprising all 147 substrate utilization
tests from API galleries, tests of volatile alcohols and
formate (not comprised in API galleries) together with morphological
characteristics, assays of nitrogen sources, enzyme
and other biochemical tests were utilized for a numerical
analysis. A resulting phenogram is given in Fig. l. These
phenotypical features are listed in detail in the description
below. DNA homology measurements are shown in Table
2. The acetone-methanol pigment spectra of the pigmented
strains are given in Fig. 2. The phenotypical comparison of
strains TA17, TA23 and TA24 shows a high degree of similarity
between these organisms (Fig. 1), which is confirmed
by the DNA homology measurements (Table 2). Only Lglutarate
utilization allows differentiating between strain
TA24 (positive) and both others (negative). Therefore, the
following description concerns all three strains: Small rods,
0.4 μm wide, and 0.9–1.4 μm long. Gram-negative, not
acid-fast. Motile by means of 1–3 polar flagella. poly-β-hydroxybutyric
is accumulated. A yellow, water insoluble and
acetone soluble intracellular pigment is formed, with peaks
at 430 nm and a shoulder at 400 nm and 455 (Fig. 2).
Colonies on nutrient agar are yellow pigmented, with a lobate
margin and a rough surface.
Metabolism is strictly aerobic, with oxygen as terminal
electron acceptor. No anaerobic growth with nitrate