Inhibition of protein synthesis
Inhibition of protein synthesis was performed in ATCC
700392 Helicobacter pylori strain by 35S methionine incorporation
assay by following the method of
for 72h.Thenthecellswereharvestedbycentrifugationandre-
suspended inwarmsyntheticM9mediumplusBrucellabrothand
incubated with 35S methioninefor10min.Afterincubation,the
cells werepelletedandwashedtwicewithM9medium.There-
after, itwasre-suspendedinM9mediumplusBrucellabroth
and incubatedwithandwithouttestsamplesfor48hat35 1C
in anincubatorundermicroaerophiliccondition.Controls
(Organismþ35S Methionine)withoutsamplewereincludedin
the study.