Ethyl acetate extract of ERIH-44 (Bacillus sp.) showed potent antibacterial (S. aureus, B. subtilis, E. faecalis) and antifungal (T. mentagrophytes, C. lunata) activities against tested microbes. B. subtilis isolate 259 presented the largest antibacterial spectrum, exhibiting inhibitory activity against several Gram-positive species such as Clostridium perfringens, E. faecalis, S. aureus and different Bacillus spp. [10] and [14]. Many strains of B. subtilis have been shown to be potential biocontrol agents against fungal pathogens. It was reported that the principle mechanism of this antifungal activity involved the production of antibiotics [7].