This process was done aseptically. First, the soil slurry was made by suspending 0.1 g of the collected dry soil in 10 ml distilled water. The slurry was mixed by vortexing for 2 min and four 1 in 10 fold serial dilutions were made from the slurry. These dilutions were done in duplicates (A and B).
Next, 3 ml volumes of the provided top agar were poured onto the bottom agar plates. The plates were allowed to set, after which 0.1 ml portions of each dilution, were then plated by spreading on the set chitin agar plates. All spread plates where labelled and incubated at 25 ̊C for a period of 14 days.