while 6 of them were weak positive and 8 isolates
showed no asparaginase production even after 48 h of
incubation.
The isolates were grown in broth medium in
order to check intracellular and extracellular Lasparaginase
producing abilities. Out of 35 isolates,
only 10 isolates showed extracellular production of
enzyme while 25 isolates did not show the extracellular
production of the enzyme. Since most of Lasparaginase
reported till date are intracellular, thus all
isolates were also checked for intracellular Lasparaginase
activity. Twenty seven E. coli isolates
showed the presence of intracellular L-asparaginase
(Table II).