Oedogonium was grown for 10 weeks, with 3 weeks of acclimation
to site conditions in potable water immediately after
transport, 2 weeks of acclimation to AWand 5 weeks “steady state”
during which productivity and metal bioremediation were quantified.
The Oedogonium was grown under batch conditions with all
water in each pond being harvested every 3e4 d. Harvests were
carried out by draining the ponds through a hose into fabric bags,
then spun in a domestic washing machine (7 min, 1000 rpm) to
remove water. The fresh weight (FW) of the biomass was recorded
and three 50 g sub samples of biomass dried at 60 C for 24 h. These
samples were re-weighed to calculate the fresh to dry weight (DW)
ratio from which areal productivity was calculated (g DWm2 d1).
The ponds were restocked with some of the spun biomass to reset a
stocking density at 0.5 g DWL1 and the surplus biomass was sundried
to