The second set was used for extraction of sugarcane
DNA and detection of SCWL phytoplasma by using
nested PCR with a SCWL phytoplasma 16s rRNA and
23S rRNA specific primers according to the protocol of
Hanboonsong et al. (2006). The quantification of
SCWL phytoplasma from each treatment used was also
performed by real-time PCR technique.