A Capillary Gas Chromatographic scan of the strains under investigation has been kindly
provided by the students of the Institute of Veterinary Medicine working with Dr. Busse.
Analysis of fatty acids by capillary gas chromatography can be done in the following way:
Using a capillary gas chromatographic analyzer, a high chromatographic efficiency is obtained by
using helium as a carrier gas, although hydrogen would yield better results (highly explosive). To
fully exploit the resolving power of capillary columns the sample must be applied as a narrow
band. This is commonly achieved by using the "hot needle" method, in which the sample is
withdrawn into the syringe barrel, the needle is then placed in the injection port and allows to
come to temperature before the plunger is rapidly depressed to flash vaporization of the sample.
A sample amount of 1ng/µL has been introduced into the capillary gas chromatographic analyzer;
the results of the scan are given as follows