LH-butter contained a 50% lower ash content (P < 0.05) than the
control as a result of the fastidious characteristics of L. helveticus
that requires an exogenous supply of minerals for growth
(Kandler & Weiss, 1986). Thus, L. helveticus could have utilized
minerals, such as calcium and potassium while growing in cream,
in which a lower concentration of ash was detected in LH-butter
compared to the control. The comparable (P > 0.05) concentration
of carbohydrates, protein, and solid non-fat content in LH-butter
with the control suggested that the fermentation process of
L. helveticus altered the fat composition of butter which could be
the main source of energy while growing in cream.