DPPH radical
scavenging activity was measured according to the
methods described by Cheung et al. (18) with
modification. Briefly, 0.8 mL of a 0.2 mM DPPH ethanol
solution was mixed with 0.2 mL of a takju sample. The
mixture was thoroughly mixed using vortexer (KMC-
1300V; Vision Scientific Co. Ltd., Bucheon, Korea) and
left to stand for 10 min at room temperature under dark
condition, after which the absorbance value was measured
at 520 nm (Multiskan GO Microplate; Thermo Fisher
Scientic).