1.Place a loopful of culture on the agar surface in Area 1. Flame and cool the loop and drag it rapidly several times across the surface of Area 1.
2. Reflame and cool the loop and turn the Petri dish 90o. Then touch the loop to a corner of the culture in Area 1 and drag it several times across the agar in Area 2. The loop should never enter Area 1 again.
3. Reflame and cool the loop and again turn the dish 90o. Streak Area 3 in the same manner as Area 2.