The specific objective of this study was to determine if MRI could detect some of the earliest physiological changes that accompany CI in tomato fruit. Current methods of assessing the occurrence and severity of CI are: (1) time consuming (e.g., enzyme assays, carbon dioxide and ethylene production), (2) destructive (e.g., measure- ment of ion leakage from excised tissue, firmness tests), or (3) occur only after the activation of secondary, downstream events (e.g., the CI index). These methods are time-proven and are indispensable, but there is a need for non-destructive methods with equiva- lent or better sensitivity to those currently used. MRI potentially offers such advantages and could be an important complemen- tary tool for studying incipient CI. We show that MRI can provide spatio-temporal resolution of chilling induced changes in Micro- Tom tomato fruit prior to development of downstream symptoms.
The specific objective of this study was to determine if MRI could detect some of the earliest physiological changes that accompany CI in tomato fruit. Current methods of assessing the occurrence and severity of CI are: (1) time consuming (e.g., enzyme assays, carbon dioxide and ethylene production), (2) destructive (e.g., measure- ment of ion leakage from excised tissue, firmness tests), or (3) occur only after the activation of secondary, downstream events (e.g., the CI index). These methods are time-proven and are indispensable, but there is a need for non-destructive methods with equiva- lent or better sensitivity to those currently used. MRI potentially offers such advantages and could be an important complemen- tary tool for studying incipient CI. We show that MRI can provide spatio-temporal resolution of chilling induced changes in Micro- Tom tomato fruit prior to development of downstream symptoms.
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