Fruit juices from different suppliers from Europe were obtained and analyzed by
HPLCiii. The individual standards malic, ascorbic, lactic and acetic acids were analytical
grade and dissolved in sulfuric acid solution (0.0225M).
We made solution of each standard which was weight manually and were different
concentration obtained. Before the HPLC analysis, all the samples were filtrated using a 0.45
µm Millipore filter.
For HPLC a Kontron instrument with photodiode array detector (DAD) Waters 990 was
used. The HPLC column was a LC-18 column from SupelcosilTM. The employed mobile
phase was sulfuric acid solution (pH 2.5) at a flow rate of 1ml/min. The detector was set at
210 nm.