2.3. TAG composition
Separation of the TAG species was performed on a Thermo
Finnigan Surveyor HPLC system with four solvent lines, degasser,
autosampler and Chromquest software (Thermo Electron Corporation,
Brussels, Belgium), which was coupled with an Alltech ELSD
2000ES evaporative laser light scattering detector . The column was a 15,063.0 mm Alltima HP C18
HL with 3 mm particle diameter. A precolumn with
a silica packing was used. A dichloromethane/acetonitrile gradient
was applied. The analysis was executed in duplicate and the sample
injections were performed in duplicate. Table 1 gives the TAG composition
of the used palm oil. The amount of DAGs (% area) concluded
from the HPLC results was 4.2%.