1. Add 0.3–0.6 mL of 8mM NaOH per 50–70 mg of tissue, or per 1 × 107 cells. Note: Resuspending the DNA is a mild base is highly recommended because isolated DNA does not resuspend well in water or Tris buffer.
1. Add 0.3–0.6 mL of 8mM NaOH per 50–70 mg of tissue,or per 1 × 107 cells.Note: Resuspending the DNA is a mild base is highlyrecommended because isolated DNA does notresuspend well in water or Tris buffer.