mg/L NAA and 0.5 mg/L NAA plus 1 mg/L TDZ gave the same results of survival to
SH agar medium supplemented 1 mg/L BA. However, SH medium supplemented with
1 mg/L BA plus 0.5 mg/L NAA and 1 mg/L BA not only showed the highest survival
but these medium showed the highest fresh weight (0.8494 and 0.7842 g,
respectively). The highest number of PLBs was produced on SH medium
supplemented with 4 mg/L BA plus 0.5 mg/L NAA (7.3 PLBs per explants). The
increasing amount of BA or TDZ alone and in combination with NAA can increase
PLB formation than lower amount of BA and TDZ. The highest number of shoot
formation was shown in SH agar medium supplemented with 0.1 mg/L TDZ (73.3
shoots per explant) (Figure 4.7A). The development of PLBs to shoot on SH agar
medium was shown in figure 4.8. From figure 4.7, the results showed the effective of
TDZ on shoot induction was more than BA. TDZ produced the highest numbers of
shoots at low concentration (figure 4.8). Increasing concentrations of BA and TDZ
showed the reduction of shoot formation and the combination of BA or TDZ with
NAA decrease shoot formation comparing with BA and TDZ alone. SH basal medium
and SH medium supplemented with 0.5 mg/L NAA gave the highest leaf length at
0.41 cm. While only eight of culture medium could produce root, these media
composted of SH basal medium, SH medium supplemented with 1 and 2 mg/L BA,
0.5 and 1 mg/L NAA, and the combination of BA and NAA at concentration of 1
mg/L BA plus 0.5 mg/L NAA, 4 mg/L BA plus 0.5 mg/L NAA and 1 mg/L BA plus 1
mg/L NAA (Figure 4.7A). The highest root length showed on SH basal medium (0.42
cm) and SH supplemented with 0.5 mg/L NAA (0.41 cm). High concentration of BA,
NAA and their combinations decreased root length. However, TDZ alone and in
combination with NAA could not produce roots. After 12 weeks, the color of SH agar
medium was changed from white color to brown color (Figure 4.9).