determination of H2O2, plant leaves will be grouned
with a 0.1 % trichloroacetic acid. To supernatant, potassium
phosphate buffer (pH 7.6), and potassium iodide were
added. Colour reagent was added to the samples, which
were thoroughly shaken and then incubated for 60 min in
the dark at temperature of 20–22 C. The absorbance was
measured at a wavelength of k = 390 nm in reference to a
calibration curve prepared with H2O2 solutions against the
blank.