This method may provide sensitive detection of ALT in the range of 25–150 UL−1 , and the reaction mechanism is complicated. Other electrochemical detection methods use a simpler two-step reaction mechanisms, such as the “glutamate sensors” proposed by Song et al., which apply glutamate oxidase (GluOx) to produce H2O2 from the L-glutamate produced by ALT , and quantifications of ALT can be further measured by the oxidation or reduction of H2O2