Video 1. DPM > dTrpA1 activation induces sleep, but not locomotor impairment. Groups of ten individual female flies with DPM(NP2721)> dTrpA1 (center), UAS-dTrpA1 (left) or DPM (NP2721)-GAL4 (right) were kept at 31°C for 2 hr before video recording. Flies with DPM driven dTrpA1 expression show normal locomotion when gently tapped (0:00:01), but quickly assume a stationary resting position after ∼30 s undisturbed (0:00:35), whereas control flies remain awake and continue to explore the environment. All flies were anesthetized with CO2, counted, and sorted
into groups of ten 1 day prior to video recording and kept on food at 22°C prior to heating. Flies were heated at 31°C on food for 2 hr and flipped to empty vials just prior to recording.