ThepeaksorbandsintheFTIRspectrumareduetothefunctional
groupspresentinaparticularsample.Inthepresentstudy,midFTIRre-
gionwasselectedtoidentifythefunctionalgroupsintheparticular
sample. Fig.6 showstheFTIRspectraofthedryalgaebiomassand
deoiledbiomassofthealgaespecies.Theregioninthespectrafrom
3100to2800cm−1
indicatesthepresenceoflipidsinthesample
andisduetothesymmetricalandasymmetricalstretchingvibrations
of –CH2– groups.These –CH2– groupsformthebackboneofthelipids
andshowtheabsorptionparticularlyat2927and2860cm−1
.Oncom-
paringtheintensityofthebandinthisregioninthespectraforthealgae
biomassanddeoiledbiomass,thedecreaseinintensitywasobservedin
thelatter,whichimpliesthatlipidshavebeensuccessfullyextractedout
fromthebiomass.Similarchangeswereobservedoncomparisonofthe
spectraofpondwateralgaeand Spirulina algaebiomassanddeoiled
biomass.TheFTIRspectra(Fig.7aandb)ofalgaeoil(pondwaterand
Spirulina respectively)werecomparedwiththeirrespectivebiodiesel